Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CD83 and the recovery rates were calculated by comparing the measured value to the expected amount of CD83 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
93
EDTA plasma(n=5)
90-105
98
heparin plasma(n=5)
87-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD83 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-100%
90-100%
88-98%
EDTA plasma(n=5)
86-92%
85-93%
87-97%
heparin plasma(n=5)
81-96%
83-96%
84-97%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
Q01151
Antigen
CD83
Applications
ELISA
Applications Notes
CD83 ELISA Kit allows for the in vitro quantitative determination of CD83 concentrations in serum, plasma, tissue homogenates and other biological fluids.