Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

Mfr.No.

EK1422

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.055	0.125	0.169	0.299	0.545	0.956	1.437	2.064

Description

Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine® (96 Tests). Quantitate Human NT5E in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

5'-nucleotidase;5'-NT;3.1.3.5;Ecto-5'-nucleotidase;CD73;NT5E;NT5, NTE;

Molecular Weight

63368 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: W27-K547

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD73

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	230	1941	5361	222	1912	4929
Standard deviation	15.41	79.58	387.29	18.64	101.33	359.81
CV (%)	6.7%	4.1%	7.2%	8.4%	5.3%	7.3%

Protein Function

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities. .

Background

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the exteal face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	230	235	243	260	242	11.37	4.6%
Sample 2	1941	1870	1830	1891	1883	40.01	2.1%
Sample 3	5361	4741	5455	5425	5245	293.24	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4907
Gene Name	NT5E
UniProt ID	Q6NZX3
Protein Name	Ecto-5'-nucleotidase
Sequence	Belongs to the 5'-nucleotidase family.

Applications	ELISA
Research Area	Associated Proteins,Atherosclerosis,Cardiovascular,Hypoxia,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001037	EK1422	96 wells/kit	$450		Add to Cart

Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

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