Human Cd70 Antigen - CD70 ELISA Kit

Mfr.No.

E5021Hu

Sensitivity

0.023ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD antigen CD70;CD27 ligand;CD27L;CD27-L;CD27LG;CD70;CD70 antigen;LPFS3;TNFSF7;TNLG8A;Tumor necrosis factor ligand superfamily member 7

Molecular Weight

21118

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5021Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.48	0.18	5.24
Intra-Assay	2	18	6.15	0.19	3.17
Intra-Assay	3	18	2.62	0.19	7.15

Background

Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Protocol

These standard curves of E5021Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.267	2.273	2.226
	2.279
6ng/ml	1.177	1.289	1.242
	1.402
3ng/ml	0.807	0.747	0.7
	0.687
1.5ng/ml	0.513	0.496	0.449
	0.48
0.75ng/ml	0.225	0.209	0.161
	0.192
0ng/ml	0.048	0.047	0
	0.046

Gene ID	970
UniProt ID	P32970

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106384	E5021Hu	48T	$320		Add to Cart
BHE12106384	E5021Hu	96T	$458		Add to Cart

Human Cd70 Antigen - CD70 ELISA Kit

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