Human CD47 ELISA Kit PicoKine®

Mfr.No.

EK1421

Sensitivity

<10 pg/ml

Detection Range

125 pg/ml - 8,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	125	250	500	1000	2000	4000	8000
O.D.	0.038	0.081	0.161	0.187	0.333	0.586	0.902	1.764

Description

Human CD47 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD47 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Leukocyte surface antigen CD47;Antigenic surface determinant protein OA3;Integrin-associated protein;IAP;Protein MER6;CD47;CD47;MER6;

Molecular Weight

35214 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-P139

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD47

Subcellular Localization

Cell membrane ; Multi-pass membrane protein .

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	193	888	3988	208	813	3951
Standard deviation	7.72	54.16	183.44	11.02	60.97	256.81
CV (%)	4%	6.1%	4.6%	5.3%	7.5%	6.5%

Protein Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .

Background

CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRP?). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	193	162	183	189	181	11.94	6.5%
Sample 2	888	960	1024	1007	969	52.69	5.4%
Sample 3	3988	4066	3941	3907	3975	59.64	1.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	961
Gene Name	CD47
UniProt ID	Q08722
Protein Name	Leukocyte surface antigen CD47
Tissue Specificity	Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
Sequence	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Applications	ELISA
Research Area	Cancer,Cell Type Markers,Immunology,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001036	EK1421	96 wells/kit	$750		Add to Cart

Human CD47 ELISA Kit PicoKine®

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