Human CD40/TNFRSF5 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ0702

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.01	0.077	0.147	0.221	0.419	0.997	1.62	2.394

Description

Human CD40/TNFRSF5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CD40 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Alternative Names

Tumor necrosis factor receptor superfamily member 5;B-cell surface antigen CD40;Bp50;CD40L receptor;CDw40;CD40;CD40;TNFRSF5;

Molecular Weight

30619 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-R193

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD40/TNFRSF5

Subcellular Localization

Isoform I: Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	93	325	538	101	357	529
Standard deviation	4.8	19.2	28.5	6.9	26.4	32.8
CV (%)	5.2%	5.9%	5.3%	6.8%	7.4%	6.2%

Protein Function

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.

Background

CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	958
Gene Name	CD40
UniProt ID	P25942
Protein Name	Tumor necrosis factor receptor superfamily member 5
Tissue Specificity	B-cells and in primary carcinomas.
Sequence	Contains 4 TNFR-Cys repeats.

Applications	ELISA
Research Area	Lymphoid,Myeloid,Stem Cells,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000010	EZ0702	5 plates/kit	$500		Add to Cart

Human CD40/TNFRSF5 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CD40/TNFRSF5 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Rate it now..!!