Human CD320 / 8D6A / TCblR ELISA Kit PicoKine®

Mfr.No.

EK1255

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.023	0.154	0.235	0.399	0.776	1.301	1.790	2.021

Description

Human CD320 / 8D6A / TCblR ELISA Kit PicoKine® (96 Tests). Quantitate Human CD320 in cell culture supeatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD320 antigen;8D6 antigen;FDC-signaling molecule 8D6;FDC-SM-8D6;Transcobalamin receptor;TCblR;CD320;CD320;8D6A;UNQ198/PRO224;

Molecular Weight

28991 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S36-Y229

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD320

Subcellular Localization

Membrane ; Single-pass type I membrane protein .

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1142	1266	4609	322	1142	5029
Standard deviation	14.65	72.16	271.93	17.06	81.08	326.88
CV (%)	5%	5.7%	5.9%	5.3%	7.1%	6.5%

Protein Function

Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. .

Background

CD320 (cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	293	300	313	285	297	10.28	3.4%
Sample 2	1266	1315	1451	1426	1364	76.51	5.6%
Sample 3	4609	4247	4591	4105	4388	217.95	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	51293
Gene Name	CD320
UniProt ID	Q9NPF0
Protein Name	CD320 antigen
Tissue Specificity	Expressed abundantly on follicular dendritic cells (FDCs). .

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Cell Biology,Cell Cycle,Cell Differentiation,Developmental Biology,Immunology,Organogenesis

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000855	EK1255	96 wells/kit	$750		Add to Cart

Human CD320 / 8D6A / TCblR ELISA Kit PicoKine®

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