Human CD32 PicoKine® Quick ELISA Kit

Mfr.No.

FEK1312

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.054	0.125	0.199	0.337	0.619	1.143	1.687	2.050

Description

Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FCGR2B/C in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II-c;IgG Fc receptor II-c;CDw32;Fc-gamma RII-c;Fc-gamma-RIIc;FcRII-c;CD32;FCGR2C;CD32, FCG2, IGFR2;FCGR2B

Molecular Weight

35578 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FCGR2B/C

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	122	404	1372	133	365	1478
Standard deviation	8.05	19.79	97.41	9.7	22.26	130.06
CV (%)	6.6%	4.9%	7.1%	7.3%	6.1%	8.8%

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	122	125	105	106	114	9.06	7.9%
Sample 2	404	361	357	357	369	19.84	5.3%
Sample 3	1372	1213	1353	1369	1326	66.06	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	2213, 9103
Gene Name	FCGR2B
UniProt ID	P31994, P31995
Protein Name	Low affinity immunoglobulin gamma Fc region receptor II-b/c

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001862	FEK1312	96 wells/kit	$499		Add to Cart

Human CD32 PicoKine® Quick ELISA Kit

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