Human CD32/FCGR2b/c ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1312

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.007	0.124	0.199	0.255	0.541	0.813	1.408	2.266

Description

Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FCGR2B in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA).

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32; FCGR2B; CD32, FCG2, IGFR2

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD32/FCGR2b/c

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	147	890	1865	168	904	2162
Standard deviation	6.62	32.93	80.2	9.75	41.6	112.42
CV (%)	4.5%	3.7%	4.3%	5.8%	4.6%	5.2%

Protein Function

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B- cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	2213
Gene Name	FCGR2B
UniProt ID	P31994, P31995
Tissue Specificity	Is the most broadly distributed Fc-gamma- receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunoglobulins,Immunology,Innate Immunity,Lymphoid,Macrophage/Inflammation,Myeloid,Receptors,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000045	EZ1312	5 plates/kit	$500		Add to Cart

Human CD32/FCGR2b/c ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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