Human CD226 antigen(CD226) ELISA kit

Mfr.No.

CSB-EL004901HU

Sensitivity

0.22 ng/mL

Detection Range

0.9 ng/mL-60 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

CD226 molecule, DNAM-1, DNAM1, PTA1, TLiSA1, CD226 antigen, DNAX accessory molecule-1, T lineage-specific activation antigen 1 antigen, adhesion glycoprotein, platelet and T cell activation antigen 1

Recovery

The recovery of human PAPPA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-100
EDTA plasma (n=4)	95	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PAPPA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-98
1:2	Average %	92
	Range %	88-99
1:4	Average %	94
	Range %	90-101
1:8	Average %	94
	Range %	86-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.517	2.483	2.500	2.453
25	1.294	1.328	1.311	1.264
10	0.614	0.626	0.620	0.573
4	0.311	0.299	0.305	0.258
2	0.159	0.156	0.158	0.111
0	0.046	0.047	0.047	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q15762

Applications	ELISA
Research Area	CD226

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501723	CSB-EL004901HU	96T	$695	Add to Cart
BHE10501723	CSB-EL004901HU	5x96T	$3336	Add to Cart
BHE10501723	CSB-EL004901HU	10x96T	$6602.50	Add to Cart

Human CD226 antigen(CD226) ELISA kit

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