Human CD206/CLEC13D PicoKine® Quick ELISA Kit

Mfr.No.

FEK2049

Sensitivity

<50 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.034	0.179	0.285	0.502	0.883	1.461	2.019	2.326

Description

Human CD206/CLEC13D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD206/CLEC13D in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-K1383

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human MRC1

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1214	5652	25412	1333	6144	26014
Standard deviation	75.27	356.08	1296.01	85.31	423.94	1794.97
CV(%)	6.2%	6.3%	5.1%	6.4%	6.9%	6.9%

Background

The mannose receptor (Cluster of Differentiation 206, CD206) is a C-type lectin primarily present on the surface of macrophages and immature dendritic cells, but is also expressed on the surface of skin cells such as human dermal fibroblasts and keratinocytes. It is the first member of a family of endocytic receptors that includes Endo180 (CD280), M-type PLA2R, and DEC-205 (CD205). This gene is mapped to 10p12.33. The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein tuover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1214	1347	1449	1542	1388	68.01	4.9%
Sample 2	5652	6145	5784	5912	5873	340.65	5.8%
Sample 3	25412	26874	29748	25944	26995	1754.64	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	4360
Gene Name	MRC1
UniProt ID	P22897

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000282	FEK2049	96 wells/kit	$499		Add to Cart

Human CD206/CLEC13D PicoKine® Quick ELISA Kit

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Reproducibility

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