Human CD164 ELISA Kit PicoKine®

Mfr.No.

EK1811

Sensitivity

<50 pg/ml

Detection Range

93.8 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.000	0.082	0.173	0.294	0.522	0.976	1.585	2.118

Description

Human CD164 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD164 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Sialomucin core protein 24; MUC-24; Endolyn; Multi-glycosylated core protein 24; MGC-24; MGC-24v; CD164

Immunogen

Expression system for standard: NS0; Immunogen sequence: D24-D162

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CD164

Subcellular Localization

Cell membrane, Single-pass type I membrane protein, Endosome membrane, Lysosome membrane, Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	149	717	2964	162	741	2982
Standard deviation	8.2	29.4	168.95	10.04	48.17	217.7
CV (%)	5.5%	4.1%	5.7%	6.2%	6.5%	7.3%

Protein Function

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38 (lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediat

Background

Sialomucin core protein 24 also known as endolyn or CD164? is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	149	135	144	154	145	7.01	4.8%
Sample 2	717	672	786	731	726	40.68	5.6%
Sample 3	2964	3060	3039	3034	3049	62.59	2.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8763
Gene Name	CD164
UniProt ID	Q04900
Tissue Specificity	Isoform 1 and isoform 3 are expressed in hematopoietic and non-hematopoietic tissues. Isoform 1 is expressed by prostate cancer tumors and prostate cancer cell lines. The expression is greater in bone metastases than in primary tumors. Expression in osseo

Applications	ELISA
Research Area	Extracellular Matrix,Signal Transduction,Tags & Cell Markers,Tumor Associated,Tumor Immunology,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001404	EK1811	96 wells/kit	$750		Add to Cart

Human CD164 ELISA Kit PicoKine®

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