Human CD160 antigen(CD160) ELISA kit

Human CD160 antigen(CD160) ELISA kit

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Mfr.No.

CSB-EL004881HU

Alternative Names

CD160 molecule, BY55, FLJ46513, NK1, NK28, CD160 antigen, CD160 transmembrane isoform, OTTHUMP00000015585, natural killer cell receptor, immunoglobulin superfamily member

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	101	95-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	87
	Range %	83-94
1:10	Average %	97
	Range %	92-102
1:20	Average %	105
	Range %	98-111
1:40	Average %	93
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.245	2.264	2.255	2.072
100	1.805	1.855	1.830	1.647
50	1.178	1.155	1.167	0.984
25	0.739	0.796	0.768	0.585
12.5	0.398	0.421	0.410	0.227
6.25	0.312	0.335	0.324	0.141
3.12	0.259	0.248	0.254	0.071
0	0.188	0.178	0.183	?

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	O95971
Antigen	CD160 molecule

Applications

ELISA

Research Area

CD160

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501718	CSB-EL004881HU	96T	$695	Add to Cart
BHE10501718	CSB-EL004881HU	5x96T	$3336	Add to Cart
BHE10501718	CSB-EL004881HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.