Human CD109 antigen(CD109) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL004878HU

Alternative Names

CD109 molecule, CPAMD7, DKFZp762L1111, FLJ38569, FLJ41966, RP11-525G3.1, CD109 antigen, Gov platelet alloantigens, activated T-cell marker CD109

Reactivity

Human

Detection Range

94 pg/mL-6000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	80-86
EDTA plasma (n=4)	96	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	87
	Range %	83-90
1:40	Average %	100
	Range %	97-103
1:80	Average %	96
	Range %	90-102
1:160	Average %	90
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

23.4 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q6YHK3
Antigen	CD109 molecule

Applications

ELISA

Research Area

CD109

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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