Human CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0331

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1,000	2,000
O.D.	0.001	0.242	0.448	0.855	1.494	2.270	2.488	2.473

Description

Human CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CCL24/Eotaxin-2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V27-C119

Kit Components

Description Quantity Volume
Anti-tag Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
Antibody A 1 3ml
Antibody B 1 3ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human CCL24

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	79	211	864	76	231	787
Standard deviation	3.87	12.87	47.52	3.65	15.94	46.43
CV (%)	4.9%	6.1%	5.5%	4.8%	6.9%	5.9%

Background

Chemokine(C-C motif) ligand 24(CCL24) also known as myeloid progenitor inhibitory factor 2(MPIF-2) or eosinophil chemotactic protein 2(eotaxin-2) is a protein that in humans is encoded by the CCL24 gene. By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	79	88	76	69	78	3.74	4.8%
Sample 2	211	190	202	206	202	7.69	3.8%
Sample 3	864	1004	899	921	922	67.31	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	6369
Gene Name	CCL24
UniProt ID	O00175

Applications	ELISA

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000308	FEK0331	96 wells/kit	$499		Add to Cart

Human CCL24/Eotaxin-2 PicoKine® Quick ELISA Kit

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Reproducibility

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