Human CCL23/MPIF-1 ELISA Kit PicoKine®

Mfr.No.

EK1224

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.4	125	250	500	1,000	2,000
O.D.	0.095	0.132	0.159	0.232	0.337	0.568	1.079	1.882

Description

Human CCL23/MPIF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL23 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 23;CK-beta-8;CKB-8;Macrophage inflammatory protein 3;MIP-3;Myeloid progenitor inhibitory factor 1;MPIF-1;Small-inducible cytokine A23;CCL23 (19-99);CCL23 (22-99);CCL23 (27-99);CCL23 (30-99);CCL23;MIP3, MPIF1, SCYA23;

Molecular Weight

13411 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R22-N120

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CCL23

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	83	239	875	85	246	848
Standard deviation	9%%2	18.16	66.5%5	5.52	22.14	78.86
CV (%)	9%%	7.6%	7.8%	6.5%	9%	9.3%

Protein Function

Shows chemotactic activity for monocytes, resting T- lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are more potent chemoattractants than the small-inducible cytokine A23. .

Background

Chemokine (C-C motif) ligand 23 (CCL23), also known as MIP-3 and MPIF-1, is a small cytokine belonging to the CC chemokine family. It is mapped to 17q12. CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. MPIF1 has chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. Moreover, CCL23 is a ligand for the chemokine receptor CCR1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	83	91	90	90	88	3.2	3.6%
Sample 2	239	230	235	226	232	4.92	2.1%
Sample 3	875	855	806	770	826	41.16	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6368
Gene Name	CCL23
UniProt ID	P55773
Protein Name	C-C motif chemokine 23
Tissue Specificity	High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23 (19-99), CCL23 (22-99), CCL23 (27-99), CCL23 (30-99) are found in high levels in synovial fluids from rheumatoid patients. .
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Chemokines,G Protein Signaling,Immunology,Innate Immunity,Macrophage/Inflammation,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000836	EK1224	96 wells/kit	$499		Add to Cart

Human CCL23/MPIF-1 ELISA Kit PicoKine®

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