Matrices listed below were spiked with certain level of CCK and the recovery rates were calculated by comparing the measured value to the expected amount of CCK in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
99
EDTA plasma(n=5)
88-102
95
heparin plasma(n=5)
87-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CCK ELISA Kit allows for the in vitro quantitative determination of CCK concentrations in serum, plasma, tissue homogenates and other biological fluids.