Matrices listed below were spiked with certain level of CCDC83 and the recovery rates were calculated by comparing the measured value to the expected amount of CCDC83 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
83-102
96
EDTA plasma(n=5)
87-102
93
heparin plasma(n=5)
84-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCDC83 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-99%
83-105%
93-105%
EDTA plasma(n=5)
91-100%
91-97%
93-98%
heparin plasma(n=5)
90-98%
91-100%
90-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q8IWF9
Applications
ELISA
Applications Notes
CCDC83 ELISA Kit allows for the in vitro quantitative determination of CCDC83 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CCDC83(Coiled-coil domain-containing protein 83) ELISA Kit