Matrices listed below were spiked with certain level of CCDC25 and the recovery rates were calculated by comparing the measured value to the expected amount of CCDC25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
85-103
95
heparin plasma(n=5)
86-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCDC25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-98%
85-104%
92-102%
EDTA plasma(n=5)
85-97%
84-94%
82-101%
heparin plasma(n=5)
86-98%
88-98%
80-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q86WR0
Applications
ELISA
Applications Notes
CCDC25ELISA Kit allows for the in vitro quantitative determination of CCDC25 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CCDC25(Coiled-coil domain-containing protein 25)ELISA Kit