Human Cathepsin V/CTSV ELISA Kit PicoKine®

Mfr.No.

EK1929

Sensitivity

<15 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.096	0.132	0.216	0.379	0.609	1.011	1.828	2.542

Description

Human Cathepsin V ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSV in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cathepsin L2; Cathepsin U; Cathepsin V; CTSV; CATL2; CTSL2; CTSU; UNQ268/PRO305

Immunogen

Expression system for standard: NS0; Immunogen sequence: V18-V334

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cathepsin V

Subcellular Localization

Lysosome

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	100	714	2346	136	823	2464
Standard deviation	5.30	44.27	105.57	6.39	52.67	133.06
CV (%)	5.3%	6.2%	4.5%	4.7%	6.4%	5.4%

Protein Function

Cysteine protease. May have an important role in coeal physiology.

Background

Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in coeal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alteatively spliced variants, encoding the same protein, have been identified.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	100	121	111	116	112	7.77	6.9%
Sample 2	741	756	857	851	801	53.05	6.6%
Sample 3	2346	2384	2464	2369	2390	44.40	1.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1515
Gene Name	CTSV
UniProt ID	O60911
Tissue Specificity	Predominantly expressed in the thymus and testis. Also expressed in coeal epithelium, and to a lesser extent in conjunctival epithelium and skin.

Applications	ELISA
Research Area	Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001441	EK1929	96 wells/kit	$750		Add to Cart

Human Cathepsin V/CTSV ELISA Kit PicoKine®

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