Human Cathepsin S (CTSS) ELISA Kit

Mfr.No.

CSB-E13722h

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL- 4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

cathepsin S, Cathepsin S ELISA Kit; CathepsinS ELISA Kit; CATS_HUMAN ELISA Kit; Ctss ELISA Kit

Recovery

The recovery of pig ?-TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	95	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig ?-TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	96
	Range %	91-100
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P25774

Applications	ELISA
Research Area	CTSS

Storage	Store at 2-8°C. Please refer to protocol.

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