Human Cathepsin S - CATH-S ELISA Kit

Mfr.No.

E0712Hu

Sensitivity

14.51ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Cathepsin S (also known as Cath-S) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cathepsin S;CTSS

Molecular Weight

37496

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0712Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2538.6	136.75	5.4
Intra-Assay	2	18	449.1	30.92	6.9
Intra-Assay	3	18	1014.4	72.37	7.1

Background

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules and MHC class II antigen presentation (PubMed: 30612035). The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.

Protocol

These standard curves of E0712Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.212	2.16	2.111
	2.109
2400ng/L	1.714	1.738	1.689
	1.763
1200ng/L	1.085	1.115	1.066
	1.146
600ng/L	0.724	0.719	0.67
	0.715
300ng/L	0.434	0.435	0.385
	0.436
0ng/L	0.05	0.05	0
	0.049

Gene ID	1520
UniProt ID	P25774

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102392	E0712Hu	96T	$458		Add to Cart
BHE12102392	E0712Hu	48T	$320		Add to Cart

Human Cathepsin S - CATH-S ELISA Kit

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