Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CTSG Ab and the recovery rates were calculated by comparing the measured value to the expected amount of CTSG Ab in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
86-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTSG Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-96%
86-103%
87-104%
EDTA plasma(n=5)
87-101%
89-101%
86-93%
heparin plasma(n=5)
81-98%
82-97%
80-98%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Cathepsin G Antibody ELISA kit allows for the in vitro quantitative determination of CTSG Ab concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Cathepsin G Antibody (CTSG Ab) ELISA kit