Human Cathepsin B ELISA Kit PicoKine®

Mfr.No.

EK0670

Sensitivity

<5 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.048	0.217	0.299	0.522	0.897	1.074	1.548	1.923

Description

Human Cathepsin B ELISA Kit PicoKine® (96 Tests). Quantitate Human CTSB in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB;

Molecular Weight

37822 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: R18-I339

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cathepsin B

Subcellular Localization

Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	252	1856	4296	234	1923	4064
Standard deviation	17.64	129.92	326.49	17.85	148.07	377.95
CV (%)	7%	7%	7.6%	7.8%	7.7%	9.3%

Protein Function

Thiol protease which is believed to participate in intracellular degradation and tuover of proteins. Has also been implicated in tumor invasion and metastasis.

Background

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	252	275	235	272	258	16.19	6.2%
Sample 2	1856	1776	1691	1826	1787	62.48	3.4%
Sample 3	4296	4210	3832	4617	4238	279.56	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1508
Gene Name	CTSB
UniProt ID	P07858
Protein Name	Cathepsin B
Sequence	Belongs to the peptidase C1 family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cell Adhesion,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000556	EK0670	96 wells/kit	$399		Add to Cart

Human Cathepsin B ELISA Kit PicoKine®

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