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Home / ELISA Kit

Human Caspase 1(Casp-1) ELISA Kit

SKU : BHE10501675

This Mouse Coagulation factor VIII (F8) ELISA Kit was designed for the quantitative measurement of Mouse Coagulation factor VIII (F8) protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E13025h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of mouse F? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		86		80-92
EDTA plasma (n=4)		92		88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse F? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1000		Average %		95
		Range %		89-99
1:2000		Average %		96
		Range %		92-101
1:4000		Average %		99
		Range %		94-102
1:8000		Average %		93
		Range %		86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.564	2.487	2.526	2.381
10	2.117	2.254	2.186	2.041
5	1.575	1.667	1.621	1.476
2.5	1.065	1.074	1.070	0.925
1.25	0.605	0.622	0.614	0.469
0.625	0.332	0.344	0.338	0.193
0.312	0.238	0.232	0.235	0.090
0	0.146	0.144	0.145

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	P29466

Applications	ELISA
Research Area	CASP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501675	CSB-E13025h	96T	$790		Add to Cart
BHE10501675	CSB-E13025h	5x96T	$3792		Add to Cart
BHE10501675	CSB-E13025h	10x96T	$7505		Add to Cart

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