Human Casein Kinase I Isoform Epsilon

Mfr.No.

E6613Hu

Sensitivity

4.99ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Casein kinase I isoform epsilon;CKIe;CKIepsilon;CKI-epsilon;CSNK1E;HCKIE;LOC400927-CSNK1E

Molecular Weight

47315

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6613Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	258.32	11.4	4.41
Intra-Assay	2	18	736.42	21.86	2.97
Intra-Assay	3	18	611.25	26.98	4.41

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1 and DVL2. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.

Protocol

These standard curves of E6613Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.762	1.752	1.696
	1.741
600ng/L	0.986	1.038	0.982
	1.09
300ng/L	0.563	0.545	0.489
	0.528
150ng/L	0.222	0.183	0.127
	0.144
75ng/L	0.151	0.108	0.052
	0.065
0ng/L	0.063	0.056	0
	0.049

Gene ID	102800317, 1454
UniProt ID	P49674

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115334	E6613Hu	48T	$320		Add to Cart
BHE12115334	E6613Hu	96T	$458		Add to Cart

Human Casein Kinase I Isoform Epsilon - CSNK1E ELISA Kit

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