Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

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Mfr.No.

CSB-EL005898HU

Sensitivity

7.8 mU/mL

Detection Range

31.25 mU/mL-2000 mU/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

carboxypeptidase N, polypeptide 1, CPN, FLJ40792, SCPN, anaphylatoxin inactivator, arginine carboxypeptidase, carboxypeptidase N catalytic subunit, carboxypeptidase N polypeptide 1 50 kD, carboxypeptidase N small subunit, kininase I, lysin

Recovery

The recovery of human MG7-Ag spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	93	88-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MG7-Ag in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	96
	Range %	91-105
1:4	Average %	95
	Range %	87-102
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average
15	2.048	2.027	2.038
10	1.392	1.388	1.390
4.5	0.728	0.731	0.730
1.5	0.304	0.307	0.306

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15169

Applications	ELISA
Research Area	CPN1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501639	CSB-EL005898HU	96T	$695	Add to Cart
BHE10501639	CSB-EL005898HU	5x96T	$3336	Add to Cart
BHE10501639	CSB-EL005898HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<20%

Three samples of known concentration were tested in twenty assays to assess.