Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit

Human Carbonyl reductase [NADPH] 1 (CBR1/CBR/CRN) ELISA kit

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Mfr.No.

CSB-E17009h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

carbonyl reductase 1, CBR, SDR21C1, hCBR1, carbonyl reductase (NADPH) 1, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, short chain dehydrogenase/reductase family 21C, member 1

Recovery

The recovery of human FA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	89
	Range %	84-93
1:40	Average %	93
	Range %	89-97
1:80	Average %	95
	Range %	90-100
1:160	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.093	0.090	0.092
100	0.227	0.208	0.218
50	0.378	0.367	0.373
25	0.618	0.637	0.628
12.5	0.986	1.003	0.995
0	1.485	1.429	1.457

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P16152

Applications	ELISA
Research Area	CBR1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501632	CSB-E17009h	96T	$790	Add to Cart
BHE10501632	CSB-E17009h	5x96T	$3792	Add to Cart
BHE10501632	CSB-E17009h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.