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Home / ELISA Kit

Human Carbonhydrate Antigen 125(CA125) ELISA Kit

SKU : BHE10501622

This Human HLA-DRA ELISA Kit was designed for the quantitative measurement of Human HLA-DRA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL .

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E04771h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human HLA-DRA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-96
EDTA plasma (n=4)		96		90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HLA-DRA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		91
		Range %		84-98
1:2		Average %		91
		Range %		85-95
1:4		Average %		96
		Range %		92-102
1:8		Average %		103
		Range %		96-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.247	2.235	2.241	2.121
600	1.492	1.589	1.541	1.421
300	0.974	0.999	0.987	0.867
150	0.622	0.632	0.627	0.507
75	0.432	0.412	0.422	0.302
37.5	0.289	0.310	0.300	0.180
18.75	0.208	0.198	0.203	0.083
0	0.121	0.119	0.120	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	Q8WXI7

Applications	ELISA
Research Area	CA125

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501622	CSB-E04771h	96T	$450		Add to Cart
BHE10501622	CSB-E04771h	5x96T	$2160		Add to Cart
BHE10501622	CSB-E04771h	10x96T	$4275		Add to Cart

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