Human Carbohydrate Sulfotransferase 8

Mfr.No.

E2877Hu

Sensitivity

9.98ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Carbohydrate sulfotransferase 8;CHST8;GalNAc-4-O-sulfotransferase 1;GalNAc4ST;GALNAC4ST1;GalNAc4ST-1;GalNAc-4-ST1;N-acetylgalactosamine-4-O-sulfotransferase 1;PSS3;

Molecular Weight

48834

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2877Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	995.85	44.18	4.44
Intra-Assay	2	18	1443.07	39.17	2.71
Intra-Assay	3	18	229.57	14.42	6.28

Background

Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Required for biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Only active against terminal GalNAcbeta1,GalNAcbeta. Not active toward chondroitin.

Protocol

These standard curves of E2877Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.453	2.351	2.296
	2.249
1200ng/L	1.334	1.36	1.305
	1.386
600ng/L	0.776	0.797	0.742
	0.819
300ng/L	0.532	0.487	0.432
	0.442
150ng/L	0.311	0.294	0.239
	0.277
0ng/L	0.056	0.055	0
	0.054

Gene ID	64377
UniProt ID	Q9H2A9

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104414	E2877Hu	48T	$320		Add to Cart
BHE12104414	E2877Hu	96T	$458		Add to Cart

Human Carbohydrate Sulfotransferase 8 - CHST8 ELISA Kit

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