Human Carbohydrate Response Element Binding Protein

Mfr.No.

E6665Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

bHLHd14;Carbohydrate-responsive element-binding protein;CHREBP;Class D basic helix-loop-helix protein 14;MIO;MLX;MLX interactor;MLX-interacting protein-like;MONDOB;WBSCR14;Williams-Beuren syndrome chromosomal region 14 protein;WS basic-helix-loop-helix leucine zipper protein;WS-bHLH;

Molecular Weight

93073

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6665Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.51	0.24	4.36
Intra-Assay	2	18	7.08	0.2	2.79
Intra-Assay	3	18	1.76	0.08	4.45

Background

Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3' (By similarity).

Protocol

These standard curves of E6665Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.544	2.6	2.553
	2.656
8ng/ml	1.544	1.494	1.447
	1.444
4ng/ml	0.897	0.914	0.867
	0.931
2ng/ml	0.415	0.421	0.374
	0.426
1ng/ml	0.203	0.208	0.161
	0.212
0ng/ml	0.036	0.047	0
	0.058

Gene ID	51085
UniProt ID	Q9NP71

Research Area	Epigenetics and Nuclear Signaling; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115315	E6665Hu	48T	$320		Add to Cart
BHE12115315	E6665Hu	96T	$458		Add to Cart

Human Carbohydrate Response Element Binding Protein - CHREBP ELISA Kit

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