Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Mfr.No.

EK2287

Sensitivity

<2 pmol/L

Detection Range

15.6 pmol/L - 1,000 pmol/L

Reactivity

Human, Canine, Porcine

Sample Volume

Concentration(pmol/L)	0	15.6	31.2	62.5	125	250	500	1,000
O.D.	0.046	0.084	0.197	0.281	0.461	0.888	1.251	2.012

Description

Human/Canine/Porcine Insulin ELISA Kit PicoKine® (96 Tests). Quantitate Human/canine/porcine INS in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 2 pmol/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F25-N110

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pmol/L)	28	93	474	25	118	274
Standard deviation	1.53	2.72	10.57	1.14	5.17	21.81
CV(%)	5.5%	2.9%	2.2%	4.5%	4.4%	8%

Background

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Reproducibility

Lots	Lot 1 (pmol/L)	Lot 2 (pmol/L)	Lot 3 (pmol/L)	Lot 4 (pmol/L)	Mean (pmol/L)	Standard Deviation	CV (%)
Sample 1	28	18	24	19	22	1.69	7.6%
Sample 2	93	93	112	85	96	6.96	7.3%
Sample 3	474	411	299	444	407	21.08	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	3630
Gene Name	INS
UniProt ID	P01308

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001836	EK2287	96 wells/kit	$499		Add to Cart

Human/Canine/Porcine Insulin ELISA Kit PicoKine®

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