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Home / ELISA Kit

Human Canalicular multispecific organic anion transporter 1 (ABCC2) ELISA Kit

SKU : BHE13703075

ABCC2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).his protein is a member of the MRP subfamily, which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells.Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

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Mfr.No.	KTE60859
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Canalicular multispecific organic anion transporter 1 (ABCC2) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC2.
Alternative Names	ABCC2; ABC30; CMOAT; DJS; KIAA1010; MRP2; cMRP; canalicular multispecific organic anion transporter
Detection Method	Colorimetric
Kit Components	Human Canalicular multispecific organic anion transporter 1 microplate Human Canalicular multispecific organic anion transporter 1 standard Human Canalicular multispecific organic anion transporter 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Canalicular multispecific organic anion transporter 1 (ABCC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCC2. No significant cross-reactivity or interference between Human ABCC2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ABCC2 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White).his protein is a member of the MRP subfamily, which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells.Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.
Notes	This Human Canalicular multispecific organic anion transporter 1 (ABCC2) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC2 in samples. An antibody specific for ABCC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1244

Applications	ELISA
Applications Notes	This Human Canalicular multispecific organic anion transporter 1 (ABCC2) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC2 in samples. An antibody specific for ABCC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703075	KTE60859	48 T	$339		Add to Cart
BHE13703075	KTE60859	96 T	$589		Add to Cart
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BHE13703075	KTE60859	96 Tx50	$24569		Add to Cart

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