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Home / ELISA Kit

Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit

SKU : BHE13703369

Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit.
The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is colinear with the PRKACA gene. Thus, the authors concluded that the PRKACG gene is a PRKACA-derived retroposon. Northern blot analysis detected PRKACG expression in fractionated germ cells of human testes.Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta.

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Mfr.No.	KTE61153
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACG.
Alternative Names	PRKACG; KAPG; PKACg; PKA C-gamma; serine(threonine) protein kinase
Detection Method	Colorimetric
Kit Components	Human CAMP-dependent protein kinase catalytic subunit gamma microplate Human CAMP-dependent protein kinase catalytic subunit gamma standard Human CAMP-dependent protein kinase catalytic subunit gamma detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKACG. No significant cross-reactivity or interference between Human PRKACG and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Cyclic AMP-dependent protein kinase (PKA) consists of two catalytic subunits and a regulatory subunit dimer. This gene encodes the gamma form of its catalytic subunit. The gene is intronless and is thought to be a retrotransposon derived from the gene for the alpha form of the PKA catalytic subunit. The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is colinear with the PRKACA gene. Thus, the authors concluded that the PRKACG gene is a PRKACA-derived retroposon. Northern blot analysis detected PRKACG expression in fractionated germ cells of human testes.Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta.
Notes	This Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACG in samples. An antibody specific for PRKACG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5568

Applications	ELISA
Applications Notes	This Human CAMP-dependent protein kinase catalytic subunit gamma (PRKACG) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACG in samples. An antibody specific for PRKACG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703369	KTE61153	48 T	$339		Add to Cart
BHE13703369	KTE61153	96 T	$589		Add to Cart
BHE13703369	KTE61153	96 Tx5	$2629		Add to Cart
BHE13703369	KTE61153	96 Tx50	$24569		Add to Cart

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