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Home / ELISA Kit

Human CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit

SKU : BHE13703370

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.

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Mfr.No.	KTE61154
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACB.
Alternative Names	PRKACB; RP11-82H13.1; DKFZp781I2452; MGC41879; MGC9320; PKACB; PKA C-beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab; cAMP-dependent protein kinase catalytic subunit beta; protein kinase
Detection Method	Colorimetric
Kit Components	Human CAMP-dependent protein kinase catalytic subunit beta microplate Human CAMP-dependent protein kinase catalytic subunit beta standard Human CAMP-dependent protein kinase catalytic subunit beta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKACB. No significant cross-reactivity or interference between Human PRKACB and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the AMP-activated protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PRKACB is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Notes	This Human CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACB in samples. An antibody specific for PRKACB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5567

Applications	ELISA
Applications Notes	This Human CAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKACB in samples. An antibody specific for PRKACB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKACB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKACB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKACB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703370	KTE61154	48 T	$339		Add to Cart
BHE13703370	KTE61154	96 T	$589		Add to Cart
BHE13703370	KTE61154	96 Tx5	$2629		Add to Cart
BHE13703370	KTE61154	96 Tx50	$24569		Add to Cart

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