Matrices listed below were spiked with certain level of CAMK2B and the recovery rates were calculated by comparing the measured value to the expected amount of CAMK2B in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-99
94
EDTA plasma(n=5)
88-100
96
heparin plasma(n=5)
86-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CAMK2B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
86-101%
85-103%
EDTA plasma(n=5)
82-99%
83-101%
85-98%
heparin plasma(n=5)
81-99%
82-94%
80-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q13554
Antigen
CAMK2B
Applications
ELISA
Applications Notes
CAMK2B ELISA Kit allows for the in vitro quantitative determination of CAMK2B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CAMK2B(Calcium/calmodulin-dependent protein kinase type II subunit beta) ELISA Kit