Matrices listed below were spiked with certain level of S100A8/S100A9 Heterodimer and the recovery rates were calculated by comparing the measured value to the expected amount of S100A8/S100A9 Heterodimer in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-99
92
EDTA plasma(n=5)
86-102
92
heparin plasma(n=5)
89-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100A8/S100A9 Heterodimer and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
calprotectinELISA Kit allows for the in vitro quantitative determination of S100A8/S100A9 Heterodimer concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human calprotectin (S100A8/S100A9 Heterodimer) ELISA Kit