Human Calponin-2(CNN2) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL005656HU

Alternative Names

calponin 2, calponin H2, smooth muscle, neutral calponin, calponin 2, calponin H2, smooth muscle, neutral calponin

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	94	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	96
	Range %	90-101
1:4	Average %	99
	Range %	94-105
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q99439
Antigen	Calponin 2

Applications

ELISA

Research Area

CNN2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501599	CSB-EL005656HU	96T	$695	Add to Cart
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