Human Calpain-5(CAPN5) ELISA kit

Mfr.No.

CSB-EL004498HU

Alternative Names

calpain 5, FLJ46245, HTRA3, nCL-3

Reactivity

Human

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse P4HB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	99	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse P4HB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-98
1:2	Average %	103
	Range %	96-108
1:4	Average %	92
	Range %	85-97
1:8	Average %	101
	Range %	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

7.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	O15484
Antigen	calpain 5

Applications

ELISA

Research Area

CAPN5

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501596	CSB-EL004498HU	96T	$695	Add to Cart
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