Human Calpain-1 Catalytic Subunit

Mfr.No.

E4116hu

Sensitivity

0.022ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Calcium-activated neutral proteinase 1;Calpain mu-type;Calpain-1 catalytic subunit;Calpain-1 large subunit;CANP 1;CAPN 1;CAPN1;Cell proliferation-inducing gene 30 protein;Micromolar-calpain;muCANP

Molecular Weight

81890

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4116hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.2	0.29	3.5
Intra-Assay	2	18	4.9	0.21	4.3
Intra-Assay	3	18	2.8	0.15	5.3

Background

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed: 21531719, PubMed: 2400579). Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409' (PubMed: 23707407).

Protocol

These standard curves of E4116hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.426	2.276	2.243
	2.126
6ng/ml	1.416	1.379	1.346
	1.342
3ng/ml	0.682	0.67	0.637
	0.658
1.5ng/ml	0.359	0.33	0.297
	0.3
0.75ng/ml	0.181	0.179	0.146
	0.177
0ng/ml	0.047	0.033	0
	0.019

Gene ID	823
UniProt ID	P07384

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105521	E4116hu	48T	$320		Add to Cart
BHE12105521	E4116hu	96T	$458		Add to Cart

Human Calpain-1 Catalytic Subunit - CAPN1 ELISA Kit

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