Human Calcium Calmodulin-dependent Protein Kinase Type 1D

Mfr.No.

E6578Hu

Sensitivity

9.93ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Calcium/calmodulin-dependent protein kinase type 1D;CaM kinase I delta;CaM kinase ID;CaM-K1;CAMK1D;CaMKI delta;CaM-KI delta;CaMKID;CaMKI-like protein kinase;CKLiK

Molecular Weight

42914

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6578Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1134.99	41.88	3.69
Intra-Assay	2	18	489.06	34.44	7.04
Intra-Assay	3	18	941.96	57.35	6.09

Background

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta.

Protocol

These standard curves of E6578Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.402	2.266	2.199
	2.13
1200ng/L	1.383	1.395	1.328
	1.406
600ng/L	0.824	0.849	0.783
	0.874
300ng/L	0.442	0.444	0.378
	0.447
150ng/L	0.281	0.272	0.206
	0.264
0ng/L	0.061	0.067	0
	0.073

Gene ID	57118
UniProt ID	Q8IU85

Research Area	Cell Biology; Signal Transduction; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115357	E6578Hu	48T	$320		Add to Cart
BHE12115357	E6578Hu	96T	$458		Add to Cart

Human Calcium Calmodulin-dependent Protein Kinase Type 1D - CAMK1D ELISA Kit

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