Human calcitonin gene related peptide,CGRP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08210h

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

calcitonin gene related peptide,CGRP

Recovery

The recovery of human FAM132A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FAM132A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	85-89
1:2	Average %	99
	Range %	91-102
1:4	Average %	100
	Range %	93-108
1:8	Average %	94
	Range %	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10092

Applications	ELISA
Research Area	CGRP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Plasma level of calcitonin gene-related peptide in patients with polycystic ovary syndrome and its relationship to hormonal and metabolic parameters Zhe Zhang et al,Peptides,2012 Apr;34(2):343-8. Fasting serum CGRP levels are related to calcium concentrations, but cannot be elevated by short-term calcium/vitamin D supplementation. Hu F.et al,Neuropeptides.,2015 49:37-45. 3D printing mesoporous bioactive glass/sodium alginate/gelatin sustained release scaffolds for bone repair Wu J, et al,Journal of Biomaterials Applications,November 14

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