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Home / ELISA Kit

Human Calcipressin-1 (RCAN1) ELISA Kit

SKU : BHE13703033

RCAN1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. The predicted 171-amino acid protein contains 2 proline-rich regions, a putative DNA-binding domain, and an acidic region. Northern blot analysis revealed that the 2.2-kb DSCR1 transcript is expressed at the highest levels in fetal brain and adult heart and at lower levels in various other tissues. An additional 2-kb mRNA was detected in fetal and adult liver.

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Mfr.No.	KTE60817
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Calcipressin-1 (RCAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate RCAN1.
Alternative Names	RCAN1; ADAPT78; CSP1; DSC1; DSCR1; MCIP1; RCN1; Down syndrome candidate region 1; Down syndrome critical region gene 1; calcipressin 1; calcium and oxidant-inducible mRNA; modulatory calcineurin-interacting pr
Detection Method	Colorimetric
Kit Components	Human Calcipressin-1 microplate Human Calcipressin-1 standard Human Calcipressin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Calcipressin-1 (RCAN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human RCAN1. No significant cross-reactivity or interference between Human RCAN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	RCAN1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. The predicted 171-amino acid protein contains 2 proline-rich regions, a putative DNA-binding domain, and an acidic region. Northern blot analysis revealed that the 2.2-kb DSCR1 transcript is expressed at the highest levels in fetal brain and adult heart and at lower levels in various other tissues. An additional 2-kb mRNA was detected in fetal and adult liver.
Notes	This Human Calcipressin-1 (RCAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate RCAN1 in samples. An antibody specific for RCAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1827

Applications	ELISA
Applications Notes	This Human Calcipressin-1 (RCAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate RCAN1 in samples. An antibody specific for RCAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRCAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703033	KTE60817	48 T	$339		Add to Cart
BHE13703033	KTE60817	96 T	$589		Add to Cart
BHE13703033	KTE60817	96 Tx5	$2629		Add to Cart
BHE13703033	KTE60817	96 Tx50	$24569		Add to Cart

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