Human CA3 ELISA Kit PicoKine®

Mfr.No.

EK1938

Sensitivity

<50 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.035	0.066	0.111	0.199	0.380	0.671	1.105	1.803

Description

Human CA3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CA3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III; CA3

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-K260

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CA3

Subcellular Localization

Cytoplasm

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1301	6028	24574	1319	6108	24732
Standard deviation	53.34	373.74	1105.83	61.99	390.91	1335.53
CV (%)	4.1%	6.2%	4.5%	4.7%	6.4%	5.4%

Protein Function

Reversible hydration of carbon dioxide.

Background

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1301	1297	1136	1156	1222	76.83	6.2%
Sample 2	6028	5779	6552	5475	5958	394.67	6.6%
Sample 3	24574	24410	24517	21425	23731	1332.95	5.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	761
Gene Name	CA3
UniProt ID	P07451
Tissue Specificity	Muscle specific.

Applications	ELISA
Research Area	Associated Proteins,Cardiovascular,Cell Biology,Cell Type Markers,Hypoxia

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001463	EK1938	96 wells/kit	$750		Add to Cart

Human CA3 ELISA Kit PicoKine®

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