Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine®

Mfr.No.

EK0681

Sensitivity

<50 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.068	0.126	0.207	0.344	0.588	1.047	1.942	2.157

Description

Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CA2 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II; CA2

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S2-K260

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CA2

Subcellular Localization

Cell membrane, Cytoplasm

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1174	5107	21947	1365	6165	23984
Standard deviation	48.13	214.49	987.62	64.16	332.91	1367.09
CV (%)	4.1%	4.2%	4.5%	4.7%	5.4%	5.7%

Protein Function

Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Background

CA2 is a cytosolic enzyme with the highest activity among all known CAs. The carbonic anhydrases (ACs) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs relatively slowly in the absence of a catalyst. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. This gene is mapped to 8q22.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1174	1071	1108	1106	1114	37.23	3.3%
Sample 2	5107	5193	4515	4791	4901	268.69	5.4%
Sample 3	21947	21460	19663	20115	20796	937.16	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	760
Gene Name	CA2
UniProt ID	P00918

Applications	ELISA
Research Area	Alzheimer's Disease,Biochemicals,Cancer,Chemical Type,Diabetes,Heart Disease,Microbiology,Obesity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001464	EK0681	96 wells/kit	$750		Add to Cart

Human CA2 / Carbonic Anhydrase 2 ELISA Kit PicoKine®

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