Human CA125/MUC16 ELISA Kit PicoKine®

Mfr.No.

EK1471

Sensitivity

<1.5 U/ml

Detection Range

3.12 U/ml - 200 U/ml

Reactivity

Human

Sample Volume

Concentration (U/ml)	0	3.12	6.24	12.5	25	50	100	200
O.D.	0.114	0.284	0.406	0.601	0.9	1.321	1.747	2.073

Description

Human CA125/MUC16 ELISA Kit PicoKine® (96 Tests). Quantitate Human MUC16 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 1.5 U/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Mucin-16 ;MUC-16;Ovarian cancer-related tumor marker CA125 ;CA-125 ;Ovarian carcinoma antigen CA125 ;MUC16 ;CA125 ;

Molecular Weight

1519175 MW

Immunogen

natural protein

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CA125

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (U/ml)	6	8	71	4	10	77
Standard deviation	0.42	0.63	1.94	0.28	0.7	6.04
CV (%)	7%	7.9%	2.7%	7%	7%	7.8%

Protein Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. .

Background

CA-125, also known as mucin 16 or MUC16, is a protein that in humans is encoded by the MUC16 gene. MUC16 is a member of the mucin family glycoproteins. It is a component of the ocular surface, the respiratory tract and the female reproductive tract epithelia. Since MUC16 is highly glycosylated it creates a hydrophilic environment that acts as a lubricating barrier against foreign particles and infectious agents on the apical membrane of epithelial cells. Also, the cytoplasmic tail of MUC16 has been shown to interact with cytoskeleton by binding members of the ERM protein family. The expression of mucin 16 has been shown to be altered in dry eye, cystic fibrosis, and several types of cancers.

Reproducibility

Lots	Lot 1 (U/ml)	Lot 2 (U/ml)	Lot 3 (U/ml)	Lot 4 (U/ml)	Mean (U/ml)	Standard Deviation	CV (%)
Sample 1	6	7	7	7	7	0.41	6.8%
Sample 2	8	8	11	11	10	0.31	3.3%
Sample 3	71	64	98	79	78	2.56	3.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	94025
Gene Name	MUC16
UniProt ID	Q8WXI7
Protein Name	Mucin-16
Tissue Specificity	Expressed in coeal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. .

Applications	ELISA
Research Area	Signal Transduction,Tags & Cell Markers,Tumor Antigens,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001073	EK1471	96 wells/kit	$499		Add to Cart

Human CA125/MUC16 ELISA Kit PicoKine®

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