Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine®

Mfr.No.

EK0680

Sensitivity

<50 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.022	0.169	0.275	0.445	0.771	1.173	1.451	1.939

Description

Human CA1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CA1 in cell culture supeatants, cell lysates, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I; CA1

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F261

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CA1

Subcellular Localization

Cytoplasm

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	452	2590	9786	584	2656	9986
Standard deviation	18.98	157.99	636.09	31.54	114.21	559.22
CV (%)	4.2%	6.1%	6.5%	5.4%	4.3%	5.6%

Protein Function

Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.

Background

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is
mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	452	501	491	473	479	18.65	3.8%
Sample 2	2590	2841	2800	2781	2753	96.57	3.5%
Sample 3	9786	9681	9685	8581	9433	493.84	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	759
Gene Name	CA1
UniProt ID	P00915

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cell Adhesion,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001450	EK0680	96 wells/kit	$750		Add to Cart

Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine®

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