Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of C5b and the recovery rates were calculated by comparing the measured value to the expected amount of C5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
98-100
99
EDTA plasma(n=5)
90-96
94
heparin plasma(n=5)
89-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
C5b ELISA Kit allows for the in vitro quantitative determination of C5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human C5b (Complement Fragment 5b) ELISA Kit