Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of C5AR1 and the recovery rates were calculated by comparing the measured value to the expected amount of C5AR1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
96
EDTA plasma(n=5)
87-100
95
heparin plasma(n=5)
90-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5AR1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-103%
89-103%
85-101%
EDTA plasma(n=5)
83-101%
88-99%
82-101%
heparin plasma(n=5)
85-99%
88-99%
83-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P21730
Antigen
C5AR1
Applications
ELISA
Applications Notes
C5AR1 ELISA Kit allows for the in vitro quantitative determination of C5AR1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human C5AR1 (C5a anaphylatoxin chemotactic receptor 1) ELISA Kit