Human C5a(Complement Component 5a) ELISA Kit

Mfr.No.

EH0096

Alternative Names

C5a ELISA Kit, Complement Component 5a ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of C5a and the recovery rates were calculated by comparing the measured value to the expected amount of C5a in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	89-105	96
EDTA plasma(n=5)	89-105	96
heparin plasma(n=5)	88-103	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-105%	85-100%	85-102%
EDTA plasma(n=5)	83-101%	83-101%	84-98%
heparin plasma(n=5)	81-97%	83-95%	83-97%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

C5a(Complement Component 5a)

Notes

For Research Use Only

UniProt ID	P01031
Antigen	C5a

Applications	ELISA
Applications Notes	C5a ELISA Kit allows for the in vitro quantitative determination of C5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804026	EH0096	96T	$400		Add to Cart

Human C5a(Complement Component 5a) ELISA Kit

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