Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of C5 convertase and the recovery rates were calculated by comparing the measured value to the expected amount of C5 convertase in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-105
94
EDTA plasma(n=5)
90-100
96
heparin plasma(n=5)
85-101
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5 convertase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
90-95%
85-102%
87-105%
EDTA plasma(n=5)
89-95%
87-98%
85-98%
heparin plasma(n=5)
88-96%
86-100%
91-99%
Sensitivity
< 0.375ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
C5 convertase ELISA Kit allows for the in vitro quantitative determination of C5 convertase concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human C5 convertase (C5 convertase) ELISA Kit